Simultaneous determination of pramoxine HCl and benzalkonium chloride in wound care solutions by HPLCAnalytical Methods (2010)
A simple gradient reversed-phase high performance liquid chromatography (HPLC) method was developed for the simultaneous determination of total benzalkonium chloride (BAC) and pramoxine HCl in wound care solutions. Laboratory formulations were diluted 1:10 with water and injected into a HPLC system equipped with a Phenomenex, Luna CN column (100 Å, 5μm, 250 mm × 4.6 mm) in order for BAC and pramoxine to separate from other excipients and be detected by a UV detector (λ = 262 nm). The mobile phase was 0.075 mM sodium acetate trihydrate buffer (pH = 5.0) using multi-ramp gradient elution with acetonitrile. Quantitation was achieved by direct comparison of the peaks of BAC and pramoxine HCl of the sample to a reference standard of known concentrations. A stress study with acid, base, peroxide, heat, and light indicated no interference from drug product or excipients. The mean recovery results for both pramoxine and benzalkonium chloride at 100% level were 100.5 ± 0.3%, 100.5 ± 0.1% respectively (mean ± SD, n = 6). In this report, the full experimental results from developing and validating this method are presented.
Citation InformationPanagiotis Tavlarakis, Jonine Greyling and Nicholas Snow. "Simultaneous determination of pramoxine HCl and benzalkonium chloride in wound care solutions by HPLC" Analytical Methods Vol. 2 Iss. 6 (2010) p. 722 - 727
Available at: http://works.bepress.com/nicholas_snow/33/