Model for ion-exchange equilibria of macromolecules in preparative chromatography.Journal of Chromatography A (1995)
An adsorption model based on the stoichiometric displacement principle was developed for the non-linear ion-exchange of macromolecules. In this model, the adsorbed phase is a non-ideal surface solution in equilibrium with a non-ideal bulk liquid, and non-idealities in both phases are quantified in terms of activity coefficient corrections. The equilibrium model accounts for the shape and charge of the adsorbates, lateral interactions on the surface and the effects of modulating salts. Practical methods were developed for evaluating model coefficients, and the model was applied to overloaded elution chromatography of bovine serum albumin. It is shown that non-idealities in the liquid phase and lateral interactions on the surface strongly influence elution profiles.
Publication DateMay, 1995
Citation InformationYonglong Li and Neville G. Pinto. "Model for ion-exchange equilibria of macromolecules in preparative chromatography." Journal of Chromatography A Vol. 702 Iss. 1-2 (1995) p. 113 - 123
Available at: http://works.bepress.com/neville-pinto/22/