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Article
Short fibrous supports for preparative chromatographic separations of biomolecules.
Journal of Chromatography A (1992)
  • Jy-Kung King
  • Neville G. Pinto, University of Louisville
Abstract
The characteristics of short, fibrous supports for chromatographic separations of biomolecules have been studied. A non-porous, silica-based fiber with an anion-exchange functionality was developed, and the performance of this support for protein separations was determined. It was established that the short-fiber column exhibits a low pressure drop, allowing separations to be performed at elevated flow-rates. The dynamic adsorption capacity of the column for three proteins, myoglobin, ovalbumin and bovine serum albumin, have been determined as a function of solution pH and salt concentration. It has been shown that the column can be used to separate mixtures of these proteins at elevated flow-rates.
Disciplines
Publication Date
September, 1992
DOI
10.1016/0021-9673(92)80149-O
Citation Information
Jy-Kung King and Neville G. Pinto. "Short fibrous supports for preparative chromatographic separations of biomolecules." Journal of Chromatography A Vol. 609 Iss. 1-2 (1992) p. 61 - 68
Available at: http://works.bepress.com/neville-pinto/15/