Skip to main content
Contribution to Book
DNA shuffling of cytochromes P450 for indigoid pigment production
Cytochrome P450 protocols
  • Nedeljka Rosic, The University of Queensland, Australia
Document Type
Book chapter
Publication Date
1-1-2013
Abstract
DNA family shuffling is a powerful method of directed evolution applied for the generation of novel enzymes with the aim of improving their existing features or creating completely new enzyme properties. This method of evolution in vitro requires parental sequences containing a high level of sequence similarity, such as is found in family members of cytochrome P450 enzymes. Cytochromes P450 (P450s or CYPs) are capable of catalyzing a variety of chemical reactions and generating a wide range of products including dye production (e.g., pigments indigo and indirubin). Application of the method of DNA family shuffling described here has enabled us to create novel P450 enzymes and to further extend the capacity of P450 to oxidize indole, leading to pigment formation.
Citation Information

Rosic, N 2015, 'DNA shuffling of cytochromes P450 for indigoid pigment production', in IR Phillips, EA Shepherd & PR Ortiz de Montellano (eds), Cytochrome P450 protocols 3rd ed, Methods in Molecular Biology; 987, Humana Press, New York, United States, pp. 205-224. ISBN: 9781627033206

Published version available from:

http://dx.doi.org/10.1007/978-1-62703-321-3_18