Recent development of tissue engineering has been emphasized on tissue regeneration and repairing in order to solve the limitation of organ and tissue transplantation issues. Biomaterial scaffold, which plays an important role in this development, not only provides a promising alternative in order to improve the efficiency of cell transplantation in tissue engineering but also to deliver cells with growth factors and drugs into injured tissue to increase the survival of cell via drug delivery system. In this study, nanofibers were fabricated through blending of a synthetic polymer, polycaprolactone (PCL) and a natural polymer, Gelatin (Ge) using electrospinning technique. Processing parameters were optimized to determine the most suitable properties of PCL/Ge nanofibers. The surface morphology of PCL/Ge nanofibers were then characterized using Scanning Electron Microscopy (SEM). Six samples of nanofibers from different amount of gelatin mixed with 10% PCL (w/v) were successfully fabricated. Experimental results showed that 18kV of high voltage provided more homogenous and less beaded nanofibers. Meanwhile, the 0.8g of Ge in 10% PCL (w/v) was set as the maximum concentration while 0.2g of Ge in 10% PCL (w/v) was set as the minimum concentration to reduce the bead formation. © (2014) Trans Tech Publications, Switzerland.