The use of nanoparticle encapsulation for drug delivery is an emerging technique to combat the disadvantages associated with delivery of biological therapeutics. Reverse micelles (RMs) are spontaneously organizing nanoscale dispersions composed of surfactant molecules and a small volume of aqueous solution in bulk organic solvent. The present study examines their promise for successful administration of biologics. A biocompatible RM mixture composed of decyl-1-monoacylglycerol (10MAG) and lauryldimethylammonium-N-oxide (LDAO) has been shown to encapsulate a wide variety of macromolecules without perturbing their structures. This mixture shows promise for delivery of such cargo into living cells as monitored by fluorescence flow cytometry. This work highlights the use of the phase-inversion temperature method (PIT) and the solvent-displacement method to produce RMs composed of 10MAG and LDAO with variation of organic solvents, water loadings, and lipids. The RM formulations are assayed to characterize encapsulation efficiency, yeast permeability, yeast lethality, and mammalian cell cytotoxicity.