Reverse micelles are soft nanoparticles created when amphiphilic molecules surround small volumes of water in a bulk nonpolar solvent. Typically these mixtures spontaneously organize into roughly spherical complexes. RM particles are quite stable, and can be used to encapsulate small hydrophilic molecules. Under optimal conditions, they can also be used to encapsulate macromolecules without perturbing their structures. Recently, a mixture composed of decylmonoacyl glycerol (10MAG) and lauryldimethylamine-N-oxide (LDAO) has been shown to encapsulated a wide array of proteins and nucleic acids. In addition to its versatility, this mixture also has the benefit of biocompatibility. The Nucci lab is exploring the utility of this mixture for a variety of biomedical and nanotechnology applications. The purpose of the present study is to thoroughly characterize the shape and dispersity of 10MAG/LDAO RMs in the presence and absence of macromolecular cargo. Dynamic light scattering is a primary tool for this endeavor and is augmented by electron microscopy and nuclear magnetic resonance. This characterization informs further application of this mixture as a biotechnological development platform.