As higher education undergoes rapid and fundamental change, eco-educators need to be prepared to promote the inclusion of the ecological sciences in the biological sciences curriculum of the future. Here, we present an instructional alignment for an introductory ecology course, which is informed by and integrated with the Four-Dimensional Ecology Education (4DEE) Framework. Our instructional alignment was created collaboratively among faculty involved in teaching the course and emphasizes the relevance and utility of the ecological sciences. We believe that this approach positions the ecological sciences for continued success and inclusion in the biological sciences curriculum of tomorrow.