Article
Treatment with Anti-Interleukin-10 Monoclonal Antibody Enhances Early Resistance to but Impairs Complete Clearance of Listeria monocytogenes Infection in Mice
Infection and Immunity
(1994)
Abstract
Mice that received an anti-interleukin-10 (anti-IL-10) neutralizing monoclonal antibody (MAb) (SXC-1)
prior to infection with Listeria monocytogenes initially demonstrated resistance to the infection, as indicated by
reduced recovery of L. monocytogenes from their spleens and livers during the first 5 days after challenge.
Anti-IL-10 MAb-treated mice then demonstrated reduced resistance during the later stage of infection, as
indicated by persistent infection with L. monocytogenes in their livers 11 days after challenge. Aspartate
aminotransferase (AST) levels (a measure of liver damage) in the sera of control mice increased between 1 and
5 days after challenge, while anti-IL-10 MAb-treated mice maintained lower AST levels. At 7 days after
challenge, AST levels in the sera of control mice decreased as the numbers of organisms declined. In contrast,
AST levels increased as the infections persisted in anti-IL-10 MAb-treated mice. The AST levels in serum
reflected liver histopathology as anti-IL-10 MAb-treated mice exhibited fewer granulomatous lesions and less
necrosis of liver tissue than the control mice during the first 5 days after challenge. Anti-IL-10 MAb treatment
altered the expression of inflammatory cytokine mRNAs during L. monocytogenes infection. Control MAbtreated
mice exhibited increased expression of tumor necrosis factor alpha and granulocyte-macrophage
colony-stimulating factor mRNA in their livers during L. monocytogenes infection, but this increase did not
occur in anti-IL-10 MAb-treated mice. Gamma interferon mRNA expression in the livers of the control
MAb-treated mice was increased between 1 and 5 days after L. monocytogenes challenge and then decreased at
7 days after challenge. In contrast, gamma interferon mRNA expression in the livers of anti-IL-10 MAb-treated
mice was not decreased until 7 days after challenge. These results indicate that endogenous IL-10 has both
beneficial and detrimental effects on the host response to L. monocytogenes infection in mice.
Publication Date
1994
Publisher Statement
This is an article from Infection and Immunity 62 (1994): 2345. Posted with permission. Copyright 1994 American Society for Microbiology
Citation Information
R. Doug Wagner, Nancy Maroushek, James F. Brown and Charles J. Czuprynski. "Treatment with Anti-Interleukin-10 Monoclonal Antibody Enhances Early Resistance to but Impairs Complete Clearance of Listeria monocytogenes Infection in Mice" Infection and Immunity Vol. 62 Iss. 6 (1994) p. 2345 - 2353 Available at: http://works.bepress.com/nancy-boury/6/