Article
Multiplication of Listeria monocytogenes in a Murine Hepatocyte Cell Line
Infection and Immunity
(1993)
Abstract
Listeria monocytogenes was shown to invade and multiply in a murine hepatocyte cell line (ATCC TIB73).
Hemolytic and nonhemolytic L. monocytogenes strains exhibited similar abilities to invade hepatocytes, but
only hemolytic L. monocytogenes multiplied within this cell line. Microscopic evaluation of monolayers stained
with Wright stain demonstrated focal necrosis (plaques) in the hepatocyte monolayers, with large numbers of
intracellular listeriae visible within the hepatocytes that lined the margins of these plaques. Murine
recombinant interleukin-lh, human recombinant tumor necrosis factor alpha, and murine recombinant
gamma interferon did not affect the multiplication of L. monocytogenes in the hepatocytes. These data confirm
in vivo observations of the intracellular multiplication of L. monocytogenes in hepatic lesions in infected mice.
Disciplines
Publication Date
1993
Publisher Statement
This is an article from Infection and Immunity 61 (1993): 3068. Posted with permission. Copyright 1993 American Society for Microbiology
Citation Information
Steve Wood, Nancy Maroushek and Charles J. Czuprynski. "Multiplication of Listeria monocytogenes in a Murine Hepatocyte Cell Line" Infection and Immunity Vol. 61 Iss. 7 (1993) p. 3068 - 2072 Available at: http://works.bepress.com/nancy-boury/5/