Real-time cell electrophysiology using a multi-channel dielectrophoretic-dot microelectrode arrayElectrophoresis (2011)
AbstractDielectrophoresis (DEP) has been used for many years for the analysis of the electrophysiological properties of cells. However, such analyses have in the past been time-consuming, such that it can take 30min or more to collect sufficient data to make valid interpretations from a single DEP spectrum. This has limited the application of the technology to a rapid tool for non-invasive, label-free research in areas from drug discovery to diagnostics. In this paper we present the development of a programmable, multi-channel DEP system for rapid biophysical assessment of populations of biological cells. A new assay format has been developed for continuous near-real-time monitoring, using simultaneous application of up to eight alternating current electrical signals to independently addressable dot microelectrodes in an array format, allowing a DEP spectrum to be measured in 20s, with a total cycle time between measurements of 90s. To demonstrate the system, human leukaemic K562 cells were monitored after exposure to staurosporine and valinomycin. The DEP response curves showed the timing and manner in which the membrane properties changed for the actions of these two drugs at the early phase of induction. This technology shows the great potential for increasing our understanding of the role of electrophysiology in drug action, by observing the changes in electrical characteristics as they occur. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
- Characterisation Dielectrophoresis Microarrays Multi-frequency Real time staurosporine valinomycin article cell membrane cell population cell structure computer program controlled study electrophoresis electrophysiology human human cell information processing leukemia cell microarray analysis microelectrode Cell Line,
- Tumor Cell Physiological Phenomena Cytological Techniques Electrophysiological Phenomena Humans Microelectrodes Microfluidic Analytical Techniques
Publication DateSeptember, 2011
Citation InformationNahrizul Adib kadri. "Real-time cell electrophysiology using a multi-channel dielectrophoretic-dot microelectrode array" Electrophoresis Vol. 32 Iss. 18 (2011)
Available at: http://works.bepress.com/nahrizuladib_kadri/9/