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Article
Comparative effect of calcium and EDTA on arsenic uptake and physiological attributes of Pisum sativum.
International Journal of Phytoremediation
  • Marina Rafiq, COMSATS Institute of Information Technology, Pakistan
  • Muhammad Shahid, COMSATS Institute of Information Technology, Pakistan
  • Ghulam Abbas, COMSATS Institute of Information Technology, Pakistan
  • Saliha Shamshad, COMSATS Institute of Information Technology, Pakistan
  • Sana Khalid, COMSATS Institute of Information Technology, Pakistan
  • Nabeel Khan Niazi, Southern Cross University
  • Camille Dumat, Université J. Jaurès, Toulouse, France
Document Type
Article
Publication Date
1-1-2017
Peer Reviewed
Peer-Reviewed
Abstract
In this study, we determined the effect of ethylenediaminetetraacetic acid (EDTA) and calcium (Ca) on arsenic (As) uptake and toxicity to Pisum sativum. Plants were treated with three levels of As (25, 125, 250 µM) in the presence and absence of three levels of Ca (1, 5, 10 mM) and EDTA (25, 125, 250 µM). Exposure to As caused an overproduction of hydrogen peroxide (H2O2) in roots and leaves, which induced lipid peroxidation and decreased pigment contents. Application of both Ca and EDTA significantly reduced As accumulation by pea, Ca being more effective in reducing As accumulation. Both Ca and EDTA enhanced As-induced H2O2 production, but reduced lipid peroxidation. In the case of pigment contents, EDTA significantly reduced pigment contents, whereas Ca significantly enhanced pigment contents compared to As alone. The effect of As treatment in the presence and absence of EDTA and Ca was more pronounced in younger leaves compared to older leaves. The effect of amendments varied greatly with their applied levels, as well as type and age of plant organs. Importantly, due to possible precipitation of Ca-As compounds, the soils with higher levels of Ca ions are likely to be less prone to food chain contamination.
Disciplines
Citation Information

Rafiq, M, Shahid, M, Abbas, G, Shamshad, S, Khalid, S, Niazi, NK, & Dumat, C 2017, 'Comparative effect of calcium and EDTA on arsenic uptake and physiological attributes of Pisum sativum' International Journal of Phytoremediation,
vol. 19, no. 7, pp. 662-669.

Published version available from:

http://dx.doi.org/10.1080/15226514.2016.1278426