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Characterization and in vitro immunomodulatory screening of fructo-oligosaccharides of asparagus racemosus willd
International Journal of Biological Macromolecules
  • Mayank Thakur, Centre for Phytochemistry and Pharmacology
  • Paul A Connellan, Centre fo Phytochemistry and Pharmacology
  • Myrna A Deseo, Centre for Phytochemistry and Pharmacology
  • Carol A Morris, Centre for Phytochemistry and Pharmacology
  • Werner Praznik, University of Natural and Applied Life Sciences, Austria
  • Loeppert, University of Natural and Applied Life Sciences, Austria
  • Dixit, Dr. H.S. Gour University, Sagar, India
Document Type
Article
Publication Date
1-1-2012
Peer Reviewed
Peer-Reviewed
Abstract

Asparagus racemosus Linn. (Fam. Liliaceae) is an ethno-pharmacologically acclaimed Ayurvedic medicinal plant. In the present study, aqueous extract of A. racemosus (ARC) was fractionated and screened for the polysaccharide fraction (ARP). The characterization was done by enzymatic, Size Exclusion, gas chromatography with flame ionization detector (GC–FID), high pressure anion exchange chromatography (HPAEC) and thin layer chromatographic analyses. Phyto-chemical evaluation confirmed the presence of 26.7% of 2 → 1 linked fructo-oligosaccharides (FOS). They have a degree of polymerization (DP) of nearly 7–8. Cytotoxicity evaluation on P388 cell lines was consistent with low cytotoxicity of the extracts. In vitroNatural Killer (NK) cell activity was evaluated using human peripheral blood mononuclear cells (PBMC) isolated from whole blood on a ficoll-hypaque density gradient. K562 a myeloid leukemia cell line, were used as target cells. ARC, tested over the range 0.2–50 μg/ml, showed a dose-related stimulation of NK cell activity with a peak increase of 16.9 ± 4.4% at 5.6 μg/ml. However, ARP demonstrated a higher stimulatory activity of 51.8 ± 1.2% at 25 μg/ml. The results indicate that the FOS from A. racemosus potentiates the NK cell activity and this could be an important mechanism underpinning the ‘Rasayana’ properties of this plant.

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Citation Information

Thakur, M, Connellan, p, Deseo, MA, Morris, C, Praznik, W, Dixit, VK 2012, 'Characterization and in vitro immunomodulatory screening of fructo-oligosaccharides of asparagus racemosus willd', International Journal of Biological Macromolecules, vol. 50, no. 1, pp. 77-81.

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Publisher's version of article available at http://dx.doi.org/10.1016/j.ijbiomac.2011.09.027