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Mechanisms Underlying the Dual-Mode Regulation of Microtubule Dynamics by Kip3/Kinesin-8
Molecular Cell (2011)
  • Xiaolei Su, Harvard Medical School
  • Weihong Qiu, Harvard Medical School
  • Mohan L. Gupta, Jr., University of Chicago
  • José B. Pereira-Leal, Instituto Gulbenkian de Ciência
  • Samara L. Reck-Peterson, Harvard Medical School
  • David Pellman, Harvard Medical School
The kinesin-8 family of microtubule motors plays a critical role in microtubule length control in
cells. These motors have complex effects on microtubule dynamics: they destabilize growing 
microtubules yet stabilize shrinking microtubules. The budding yeast kinesin-8, Kip3, accumulates 
on plus ends of growing but not shrinking microtubules. Here we identify an essential role of the 
tail domain of Kip3 in mediating both its destabilizing and stabilizing activities. The Kip3-tail 
promotes Kip’s accumulation at the plus ends and facilitates the destabilizing effect of Kip3.
However, the Kip3-tail also inhibits microtubule shrinkage and is required for promoting 
microtubule rescue by Kip3. These effects of the tail domain are likely to be mediated by the 
tubulin- and microtubule-binding activities that we describe. We propose a concentration- dependent 
model for the coordination of the destabilizing  nd stabilizing activities of Kip3 and
discuss its relevance to cellular microtubule organization.

Publication Date
September 2, 2011
Publisher Statement
Copyright 2011 Elsevier Inc.
Citation Information
Xiaolei Su, Weihong Qiu, Mohan L. Gupta, José B. Pereira-Leal, et al.. "Mechanisms Underlying the Dual-Mode Regulation of Microtubule Dynamics by Kip3/Kinesin-8" Molecular Cell Vol. 43 Iss. 5 (2011) p. 751 - 763
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