Skip to main content
Article
Effect of electrical stimulation on arteriogenesis and angiogenesis after bilateral femoral artery excision in the rabbit hind-limb ischemia model
Vascular and endovascular surgery
  • Imran Sheikh
  • Guennady Tchekanov
  • David Krum, Aurora Health Care
  • John Hare, Aurora Health Care
  • Mohamed Djelmami-Hani
  • Rajesh Maddikunta
  • M. Eyman Mortada, Aurora Sinai/Aurora St. Luke’s Medical Centers, Aurora Health Care
  • Pavel Karakozov
  • Iskander Baibekov
  • John Hauck
  • Tanvir Bajwa, Aurora Cardiovascular Services, Aurora Sinai/Aurora St. Luke’s Medical Centers, University of Wisconsin School of Medicine and Public Health
  • Masood Akhtar, Aurora Sinai/Aurora St. Luke’s Medical Centers, Aurora Health Care
  • Valeri S Chekanov
Aurora Affiliations

Department of Cardiac Research, Aurora Sinai/St. Luke’s Medical Centers

Publication Date
5-1-2005
Abstract
The effects of electrical stimulation (ES) on arteriogenesis (the opening of preexisting collaterals) and angiogenesis (formation of new capillaries) were studied after acute bilateral hind limb ischemia was induced via bilateral femoral artery excision in a rabbit model. The study evaluated the rabbit hind limbs' normal response to acute ischemia and to application of ES by calculating changes in arterial and capillary densities. Comparisons were made with our prior study, in which the femoral artery was unilaterally excised, as we attempted to expand on the topics of arteriogenesis and angiogenesis. Twelve adult New Zealand white rabbits were randomly assigned to 1 of 2 series. In Series 1, the control group, both femoral arteries were excised and no ES was applied. In Series 2, both femoral arteries were excised and ES was applied to the left limb. One lead was implanted into the left adductor muscle near the site of the excised left femoral artery (Series 2), and a stimulator (Thera, Medtronic, Inc, Minneapolis, MN) was implanted in a separate pocket. ES was applied at a rate of 3 V, 30 contractions per minute, beginning immediately after surgery and continuously for 1 month. Angiography was performed in all 12 rabbits 1 month after surgery to establish the anatomy of the collateral vessels and to demonstrate that the femoral artery stump continued to be an end artery. Contrast-opacified arteries (COAs) that crossed the grid's midline, and the total number of grid lines intersected by COAs, were tallied according to an established method. Capillary density was calculated as the number of capillaries per square millimeter of muscle. In Series 1, after 1 month, the number of COAs crossing the grid's midline was 4.5 +/-1.5 on the left and 4.8 +/-1.2 on the right side. In Series 2, the number of COAs crossing the grid's midline was 7.9 +/-1.8 on the left side (p
Document Type
Article
PubMed ID
15920655
Citation Information
Sheikh I, Tchekanov G, Krum D, et al. Effect of electrical stimulation on arteriogenesis and angiogenesis after bilateral femoral artery excision in the rabbit hind-limb ischemia model. Vasc Endovascular Surg. 2005 May-Jun;39(3):257-65.