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Clara cell secretory protein and phospholipase A2 activity modulate acute ventilator-induced lung injury in mice
Journal of applied physiology
  • S. Yoshikawa
  • T. Miyahara
  • S. D. Reynolds
  • B. R. Stripp
  • Mircea Anghelescu, Philadelphia College of Osteopathic Medicine
  • F. G. Eyal
  • J. C. Parker
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Lung vascular permeability is acutely increased by high-pressure and high-volume ventilation. To determine the roles of mechanically activated cytosolic PLA2 (cPLA2) and Clara cell secretory protein (CCSP), a modulator of cPLA2 activity, we compared lung injury with and without a PLA2 inhibitor in wild-type mice and CCSP-null mice (CCSP-/-) ventilated with high and low peak inflation pressures (PIP) for 2- or 4-h periods. After ventilation with high PIP, we observed significant increases in the bronchoalveolar lavage albumin concentrations, lung wet-to-dry weight ratios, and lung myeloperoxidase in both genotypes compared with unventilated controls and low-PIP ventilated mice. All injury variables except myeloperoxidase were significantly greater in the CCSP-/- mice relative to wild-type mice. Inhibition of cPLA2 in wild-type and CCSP-/- mice ventilated at high PIP for 4 h significantly reduced bronchoalveolar lavage albumin and total protein and lung wet-to-dry weight ratios compared with vehicle-treated mice of the same genotype. Membrane phospho-cPLA2 and cPLA2 activities were significantly elevated in lung homogenates of high-PIP ventilated mice of both genotypes but were significantly higher in the CCSP-/- mice relative to the wild-type mice. Inhibition of cPLA2 significantly attenuated both the phospho-cPLA2 increase and increased cPLA2 activity due to high-PIP ventilation. We propose that mechanical activation of the cPLA 2 pathway contributes to acute high PIP-induced lung injury and that CCSP may reduce this injury through inhibition of the cPLA2 pathway and reduction of proinflammatory products produced by this pathway. Copyright © 2005 the American Physiological Society.

This article was published in Journal of applied physiology, Volume 98, Issue 4, Pages 1264-1271.

The published version is available at

Copyright © 2005.

Citation Information
S. Yoshikawa, T. Miyahara, S. D. Reynolds, B. R. Stripp, et al.. "Clara cell secretory protein and phospholipase A2 activity modulate acute ventilator-induced lung injury in mice" Journal of applied physiology Vol. 98 Iss. 4 (2005) p. 1264 - 1271
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