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Article
A novel TaulacZ allele reveals a requirement for Pitx2 in formation of the mammillothalamic tract
Genesis (2012)
  • Mindy Waite, PhD, Aurora Research Institute
Abstract
The hypothalamic mammillary region is critical for spatial memory and vestibular processing. Pitx2 encodes a paired-like transcription factor that is highly expressed in the developing mammillary region and is required for subthalamic nucleus formation. Here we analyzed a loss of function Pitx2-TaulacZ knock-in allele to study the effects of Pitx2 deficiency on neuronal projections in the embryonic mammillary region. Pitx2-expressing neurons contribute axons to principal mammillary, mammillotegmental and mammillotectal tracts. Embryos with Pitx2 deficiency exhibit axonal fibers in the principal mammillary tract that are improperly bundled and disorganized, yet project caudally toward the tectum and tegmentum. Embryos with Nestin-Cre mediated conditional Pitx2 deficiency exhibit truncated mammillothalamic tracts (mtt) that fail to elongate, and reduced Pax6-positive cells at the branching point of the principal mammillary and mtt. These data suggest that Pitx2 mediates cell-autonomous and nonautonomous guidance cues necessary for mammillary collaterals destined to project to the anterior thalamus.
Keywords
  • neuroscience,
  • neural development,
  • development,
  • brain,
  • transcription factor,
  • migration,
  • differentiation,
  • hypothalamus,
  • projections
Publication Date
January, 2012
DOI
10.1002/dvg.20793
Citation Information
Mindy Waite. "A novel TaulacZ allele reveals a requirement for Pitx2 in formation of the mammillothalamic tract" Genesis Vol. 50 Iss. 1 (2012) p. 67 - 73
Available at: http://works.bepress.com/mindy-waite/2/