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In vivo real-time imaging of cutaneous hemoglobin concentration, oxygen saturation, scattering properties, melanin content, and epidermal thickness with visible spatially modulated light
Biomedical optics express
  • Xinlin Chen
  • Weihao Lin
  • Chenge Wang
  • Shaoheng Chen
  • Jing Sheng
  • Bixin Zeng
  • Min Xu, Fairfield University
Document Type
Article
Article Version
Publisher's PDF
Publication Date
1-1-2017
Abstract

We present the real-time single snapshot multiple frequency demodulation - spatial frequency domain imaging (SSMD-SFDI) platform implemented with a visible digital mirror device that is capable of imaging and monitoring dynamic turbid medium and processes over a large field of view. One challenge in quantitative imaging of biological tissue such as the skin is the complex structure rendering techniques based on homogeneous medium models to fail. To address this difficulty we have also developed a novel method that maps the layered structure to a homogeneous medium for spatial frequency domain imaging. The varying penetration depth of spatially modulated light on its wavelength and modulation frequency is used to resolve the layered structure. The efficacy of the real-time SSMD-SFDI platform and this two-layer model is demonstrated by imaging forearms of 6 healthy subjects under the reactive hyperemia protocol. The results show that our approach not only successfully decouples light absorption by melanin from that by hemoglobin and yields accurate determination of cutaneous hemoglobin concentration and oxygen saturation, but also provides reliable estimation of the scattering properties, the melanin content and the epidermal thickness in real time. Potential applications of our system in imaging skin physiological and functional states, cancer screening, and microcirculation monitoring are discussed at the end. © 2017 Optical Society of America

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© 2017 Optical Society of America]. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.

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Published Citation
Chen, X., Lin, W., Wang, C., Chen, S., Sheng, J., Zeng, B., & Xu, M. (2017). In vivo real-time imaging of cutaneous hemoglobin concentration, oxygen saturation, scattering properties, melanin content, and epidermal thickness with visible spatially modulated light. Biomedical optics express, 8(12), 5468-5482. doi:10.1364/BOE.8.005468.
DOI
10.1364/BOE.8.005468
None
Peer Reviewed
Citation Information
Xinlin Chen, Weihao Lin, Chenge Wang, Shaoheng Chen, et al.. "In vivo real-time imaging of cutaneous hemoglobin concentration, oxygen saturation, scattering properties, melanin content, and epidermal thickness with visible spatially modulated light" Biomedical optics express Vol. 8 Iss. 12 (2017)
Available at: http://works.bepress.com/min_xu/13/