The lactational performance of 30 healthy multiparous Holstein dairy cows was tested when cows were fed 0, 10, and 20% dried distillers grains with solubles (DDGS) that contained 13.6% fat on a dry matter (DM) basis. Cows fed 10% DDGS on a DM basis experienced a reduction in milk fat percentage of 0.5 percentage points, but did not perform differently in other measures than did cows not fed DDGS. When cows were fed 20% DDGS on a DM basis, every common measure of feed utilization was impacted negatively.