ADP-glucose pyrophosphorylase is the enzyme responsible for the production of ADP-glucose, which is used for the synthesis of starch in plants and glycogen in bacteria. This enzyme within our model organism Agrobacterium tumefaciens is allosterically regulated through various activators and inhibitors. Previously single mutation residues at predetermined sites of interest within the wild type of A. tumefaciens have been studied. Two of these sites Tyr 39 and Arg 75, are residues located on the allosteric binding site of the activator Fru6P. Therefore, we targeted this site with a double mutation of both residues to an alanine and observed the results.