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Article
Lung specific expression of a human mutant p53 affects cell proliferation in transgenic mice.
Transgenic Research (2008)
  • Miguel A. Villalona Calero, M.D.
Abstract
The human mutant p53(273H) has been shown in vitro to have both dominant-negative and gain-of-function properties, as well as to retain partial DNA-binding and transcriptional activation functions. We have developed a line of transgenic mice in which the human mutant p53(273H) is expressed in a lung specific manner (p53 (+/+/TG)). Crossing of the transgenic mice with p53 knockout mice led to generate mice with various genetic backgrounds. To evaluate the influence of p53 mutants in cell proliferation in mice lung tissue, we analyzed cell proliferation rate by Bromodeoxyuridine (BrdU) labeling and by expression of proliferating cell nuclear antigen (PCNA). BrdU analysis showed a
Publication Date
June, 2008
Citation Information
Miguel A. Villalona Calero. "Lung specific expression of a human mutant p53 affects cell proliferation in transgenic mice." Transgenic Research Vol. 17 Iss. 3 (2008) p. 355 - 366
Available at: http://works.bepress.com/miguel-villalonacalero/47/