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Article
Association of Antibiotic Resistance in Agricultural Escherichia coli Isolates with Attachment to Quartz
Applied and Environmental Microbiology
  • Ping Liu, Iowa State University
  • Michelle L. Soupir, Iowa State University
  • Martha R. Zwonitzer, Iowa State University
  • Bridgette Huss, Iowa State University
  • Laura R. Jarboe, Iowa State University
Document Type
Article
Publication Date
10-1-2011
DOI
10.1128/AEM.00717-11
Abstract

Surface water can be contaminated by bacteria from various sources, including manure from agricultural facilities. Attachment of these bacteria to soil and organic particles contributes to their transport through the environment, though the mechanism of attachment is unknown. As bacterial attachment to human tissues is known to be correlated with antibiotic resistance, we have investigated here the relationship between bacterial attachment to environmental particles and antibiotic resistance in agricultural isolates. We evaluated 203 Escherichia coliisolates collected from swine facilities for attachment to quartz, resistance to 13 antibiotics, and the presence of genes encoding 13 attachment factors. The genes encoding type I, EcpA, P pili, and Ag43 were detected, though none was significantly related to attachment. Quartz attachment was positively and significantly (P < 0.0038) related to combined resistance to amoxicillin/streptomycin/tetracycline/sulfamethazine/tylosin/chlortetracycline and negatively and significantly (P < 0.0038) related to combined resistance to nalidixic acid/kanamycin/neomycin. These results provide clear evidence for a link between antibiotic resistance and attachment to quartz in agricultural isolates. We propose that this may be due to encoding by the responsible genes on a mobile genetic element. Further exploration of the relationship between antibiotic resistance and attachment to environmental particles will improve the understanding and modeling of environmental transport processes, with the goal of preventing human exposure to antibiotic-resistant or virulent microorganisms.

Comments

This article is from Applied and Environmental Microbiology 77 (2011): 6945–6953, doi:10.1128/AEM.00717-11. Posted with permission.

Copyright Owner
American Society for Microbiology
Language
en
Date Available
2013-10-20
File Format
application/pdf
Citation Information
Ping Liu, Michelle L. Soupir, Martha R. Zwonitzer, Bridgette Huss, et al.. "Association of Antibiotic Resistance in Agricultural Escherichia coli Isolates with Attachment to Quartz" Applied and Environmental Microbiology Vol. 77 Iss. 19 (2011) p. 6945 - 6953
Available at: http://works.bepress.com/michelle_soupir/22/