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Analyzing Environmental Microbes for Genomic Regions Promoting Ionic Liquid Tolerance in E. coli
STAR (STEM Teacher and Researcher) Presentations
  • Ann Nguyen, California State University - East Bay, San Jose High School
  • Alison Richins, California State University - East Bay
  • Thomas Rüegg, Joint BioEnergy Institute
  • Steven Singer, Joint BioEnergy Institute, Lawrence Berkeley National Laboratory
  • Michael Thelen, Joint BioEnergy Institute, Lawrence Livermore National Laboratory
Publication Date
8-2012
Abstract

Ionic liquids (ILs) are promising as solvents to increase the efficiency of biofuel production; however, ILs are toxic to microbes used in the fermentation of liquid fuels. To engineer IL resistant biofuel hosts, environmental bacteria were screened for tolerance, and these were used to create gene libraries to test in E. coli. Future characterization of these libraries using molecular techniques will be used to identify genes that contribute IL-tolerance to transformed microbes.

Mentor
Michael P Thelen
Lab site
Lawrence Livermore National Laboratory (LLNL)
Citation Information
Ann Nguyen, Alison Richins, Thomas Rüegg, Steven Singer, et al.. "Analyzing Environmental Microbes for Genomic Regions Promoting Ionic Liquid Tolerance in E. coli" (2012)
Available at: http://works.bepress.com/michael_thelen/2/