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Article
Screening the Farallon Island Bacterial Collection for Ionic Liquid Tolerance to Create Genomic Libraries
STAR (STEM Teacher and Researcher) Presentations
  • Alison Richins, California State University - East Bay
  • Ann Nguyen, California State University - East Bay
  • Thomas Rüegg, Lawrence Livermore National Laboratory
  • Steven Singer, Lawrence Livermore National Laboratory
  • Michael Thelen, Lawrence Livermore National Laboratory
Publication Date
8-2012
Abstract

To find a gene that confers ionic liquid resistance in bacteria, environmental samples of bacteria were taken from a high salt environment in the FarallonIslands. The bacteria were grown on different media that contained either 1‐Ethyl‐3‐methylimidazolium chloride (EMiM‐Cl) or 1‐Ethyl‐3‐ methylimidazolium acetate (EMiM‐Ac) to determine which bacteria should be used to create a genomic library to screen for genes conferring ionic liquid resistance. Bacterial isolates IL 210, IL 15, CX32 AND CX 38 grow on media containing EMiM‐Cl as well as media containing EMiM‐Ac and are recommended for the creation of a gene library.

Mentor
Michael Thelen
Lab site
Lawrence Livermore National Laboratory (LLNL)
Citation Information
Alison Richins, Ann Nguyen, Thomas Rüegg, Steven Singer, et al.. "Screening the Farallon Island Bacterial Collection for Ionic Liquid Tolerance to Create Genomic Libraries" (2012)
Available at: http://works.bepress.com/michael_thelen/1/