Skip to main content
Article
Trehalose loading through the mitochondrial permeability transition pore enhances desiccation tolerance in rat liver mitochondria
Biochemica et Biophysica Acta: Biomembranes (2005)
  • Xiang-Hong Liu, Harvard Medical School
  • Alptekin Aksan, Harvard Medical School
  • Michael A Menze, University of Louisville
  • Steven C. Hand, Louisiana State University
  • Mehmet Toner, Harvard Medical School
Abstract
Trehalose has extensively been used to improve the desiccation tolerance of mammalian cells. To test whether trehalose improves desiccation tolerance of mammalian mitochondria, we introduced trehalose into the matrix of isolated rat liver mitochondria by reversibly permeabilizing the inner membrane using the mitochondrial permeability transition pore (MPTP). Measurement of the trehalose concentration inside mitochondria using high perfOrmance liquid chromatography showed that the sugar permeated rapidly into the matrix upon opening the MPTP. The concentration of intra matrix trehalose reached 0.29 mmoVmg protein (-190 mM) in 5 min. Mitochondria, with and without trehalose loaded into the matrix, were desiccated in a buffer containing 0.25 M trehalose by diffusive drying. After re hydration, the inner membrane integrity was assessed by measurement of mitochondrial membrane potential with the fluorescent probe JC 1. The results showed that following drying to similar water contents, the mitochondria loaded with trehalose had significantly higher inner membrane integrity than those without trehalose loading. These findings suggest the presence of trehalose in the mitochondrial matrix affords improved desiccation tolerance to the isolated mitochondria.
Keywords
  • Trehalose; Desiccation; Mitochondria; Permeabilization; Mitochondrial membrane potential; Mitochondrial permeability transition pore
Disciplines
Publication Date
2005
Publisher Statement
This work is available in definitive final version at Biochemica et Biophysica Acta: Biomembranes, 1717(1), 21-26.
Citation Information
Xiang-Hong Liu, Alptekin Aksan, Michael A Menze, Steven C. Hand, et al.. "Trehalose loading through the mitochondrial permeability transition pore enhances desiccation tolerance in rat liver mitochondria" Biochemica et Biophysica Acta: Biomembranes Vol. 1717 Iss. 1 (2005)
Available at: http://works.bepress.com/michael_menze/17/