Cryopreservation of Spin-Dried Mammalian CellsPLoS One (2011)
This study reports an alternative approach to achieve vitrification where cells are pre-desiccated prior to cooling to cryogenic temperatures for storage. Chinese Hamster Ovary (CHO) cells suspended in a trehalose solution were rapidly and uniformly desiccated to a low moisture content (<0.12 g of water per g of dry weight) using a spin-drying technique. Trehalose was also introduced into the cells using a high-capacity trehalose transporter (TRET1). Fourier Transform Infrared Spectroscopy (FTIR) was used to examine the uniformity of water concentration distribution in the spin-dried samples. 62% of the cells were shown to survive spin-drying in the presence of trehalose following immediate rehydration. The spin-dried samples were stored in liquid nitrogen (LN2) at a vitrified state. It was shown that following re-warming to room temperature and re-hydration with a fully complemented cell culture medium, 51% of the spin-dried and vitrified cells survived and demonstrated normal growth characteristics. Spin-drying is a novel strategy that can be used to improve cryopreservation outcome by promoting rapid vitrification.
- Mammalian Cells,
Publication DateSeptember, 2011
Citation InformationNilay Chakraborty, Michael A Menze, Jason Malsam, Alptekin Aksan, et al.. "Cryopreservation of Spin-Dried Mammalian Cells" PLoS One Vol. 6 Iss. 9 (2011)
Available at: http://works.bepress.com/michael_menze/10/