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Dynamic Model-Based Analysis of Furfural and HMF Detoxification by Pure and Mixed Batch Cultures of S. cerevisiae and S. stipitis
Biotechnology and Bioengineering (2014)
  • Timothy J Hanly
  • Michael A Henson, University of Massachusetts - Amherst
Inhibitory compounds that result from biomass hydrolysis are an obstacle to the efficient production of second-generation biofuels. Fermentative microorganisms can reduce compounds such as furfural and 5-hydroxymethyl furfural (HMF), but detoxification is accompanied by reduced growth rates and ethanol yields. In this study, we assess the effects of these furan aldehydes on pure and mixed yeast cultures consisting of a respiratory deficient mutant of Saccharomyces cerevisiae and wild-type Scheffersomyces stipites using dynamic flux balance analysis. Uptake kinetics and stoichiometric equations for the intracellular reduction reactions associated with each inhibitor were added to genome-scale metabolic reconstructions of the two yeasts. Further modification of the S. cerevisiae metabolic network was necessary to satisfactorily predict the amount of acetate synthesized during HMF reduction. Inhibitory terms that captured the adverse effects of the furan aldehydes and their corresponding alcohols on cell growth and ethanol production were added to attain qualitative agreement with batch experiments conducted for model development and validation. When the two yeasts were co-cultured in the presence of the furan aldehydes, inoculums that reduced the synthesis of highly toxic acetate produced by S. cerevisiae yielded the highest ethanol productivities. The model described here can be used to generate optimal fermentation strategies for the simultaneous detoxification and fermentation of lignocellulosic hydrolysates by S. cerevisiae and/or S. stipitis.
  • ethanol,
  • flux balance analysis,
  • lignocellulosic hydrolysate,
  • S. cerevisiae,
  • S. stipitis
Publication Date
February, 2014
Citation Information
Timothy J Hanly and Michael A Henson. "Dynamic Model-Based Analysis of Furfural and HMF Detoxification by Pure and Mixed Batch Cultures of S. cerevisiae and S. stipitis" Biotechnology and Bioengineering Vol. 111 Iss. 2 (2014)
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