The aims of the present study were to examine the movement demands of pre-season practice in National Collegiate Athletic Association (NCAA) division I college football players using portable global positioning system (GPS) technology and to assess perceived wellness associated with pre-season practice to determine if GPS-derived variables from the preceding day influence perceived wellness the following day. Twenty-nine players were monitored using GPS receivers (Catapult Innovations, Melbourne, Australia) during 20 pre-season practices. Individual observations (n=550) were divided into offensive and defensive position groups. Movement variables including low-, medium-, high-intensity, and sprint distance, player load, and acceleration and deceleration distance were assessed. Perceived wellness ratings (n=469) were examined using a questionnaire which assessed fatigue, soreness, sleep quality, sleep quantity, stress, and mood. A one-way ANOVA for positional movement demands, and multi-level regressions for wellness measures were used, followed by post-hoc testing to evaluate the relational significance between categorical outcomes of perceived wellness scores and movement variables. Results demonstrated significantly (p<0.05) greater total, high-intensity, and sprint distance, along with greater acceleration and deceleration distances for the DB and WR position groups compared to their respective offensive and defensive counterparts. Significant (p<0.05) differences in movement variables were demonstrated for individuals who responded more or less favorably on each of the six factors of perceived wellness. Data from the present study provide novel quantification of the position-specific physical demands and perceived wellness associated with college football pre-season practice. Results support the use of position-specific training and individual monitoring of college football players.