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Article
Correlation of genomic detection of feline coronavirus with various diagnostic assays for feline infectious peritonitis
Journal of Veterinary Diagnostic Investigation (1998)
  • Melissa A. Kennedy, University of Tennessee, Knoxville
  • K Brenneman
  • R K Millsaps
  • Leon N.D. Potgieter, University of Tennessee - Knoxville
Abstract

Primers for a reverse transcriptase-nested polymerase chain reaction (PCR) for feline coronavirus (FCV) in a variety of samples from cats were based on the 7a and 7b open reading frames of the genome. In a field trial 11 of 27 clinically ill cats were positive for FCV. All of 6 cats histologically negative for FCV were negative by PCR. Six of 7 cats with feline infectious peritonitis (FIP) confirmed by histopathology were positive by PCR. Three of 8 cats inconclusive for FIP by histopathology were positive by PCR; 2 of these had significant antibody titres. Four of the 5 PCR-negative cats that were tested showed insignificant antibody titres. PCR results using effusive fluid correlated well with histopathology results. Positive PCR results were more consistent with effusive fluid, followed by plasma and whole blood. It was concluded that this PCR is sensitive and specific for FCV but that serum is a poor sample for PCR and is often negative for FCV-infected cats.

Publication Date
1998
Citation Information
Melissa A. Kennedy, K Brenneman, R K Millsaps and Leon N.D. Potgieter. "Correlation of genomic detection of feline coronavirus with various diagnostic assays for feline infectious peritonitis" Journal of Veterinary Diagnostic Investigation Vol. 10 Iss. 1 (1998)
Available at: http://works.bepress.com/melissa_kennedy/15/