Presentation
An innovative method for the determination of trace zinc in seawater using micro-Sequential Injection and a novel fluorescent probe
American Geophysical Union (AGU), Association for the Sciences of Limnology and Oceanography (ASLO), The Oceanography Society (TOS)
(2012)
Abstract
Flow Injection (FI) technology has been considerably improved over the last two decades notably with the development of miniaturized, single channel discontinuous flow systems. This presentation will describe the principles and attributes of micro-Sequential Injection lab-on-valve (µSI-LOV), the latest development in FI methodology, and its application to the determination of trace Zn in seawater samples. The technique exploits the selectivity and sensitivity of FluoZin-3, a novel fluorescent probe that is used in a seawater matrix for the first time. The optimized reaction conditions and flow protocol yield a detection limit of 0.3nM Zn, high precision (RSD ≤ 2.5%) and a linear quantification range up to 40nM Zn. This is achieved without preconcentration using 50µL of indicator and 75µL of sample, an analytical cycle of 1min per sample and instrumentation that is simple, compact, fully automated and requires minimal maintenance. The applicability of µSI-LOV to open ocean determinations will be demonstrated using SAFE standards and open ocean Zn profiles collected in the Southern Ocean. The potential of µSI-LOV to shipboard and eventually in situ determinations of trace elements will also be discussed.
Disciplines
Publication Date
2012
Location
Salt Lake City, Utah
Citation Information
Maxime M. Grand, Jaromir Ruzicka and Hugo Miguel Rodrigues Oliveira. "An innovative method for the determination of trace zinc in seawater using micro-Sequential Injection and a novel fluorescent probe" American Geophysical Union (AGU), Association for the Sciences of Limnology and Oceanography (ASLO), The Oceanography Society (TOS) (2012) Available at: http://works.bepress.com/maxime-grand/23/