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Article
Rapid Communication: Genetic Linkage Mapping of the Porcine Fibroblast Growth Factor 7 (FGF7) Gene
Journal of Animal Science
  • K. A. Kelly, Iowa State University
  • N. J. Larsen, Iowa State University
  • Max F. Rothschild, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
1-1-1998
DOI
/1998.76102747x
Abstract

Source and Description of Primers. The forward primer was developed from a human fibroblast growth factor ( FGF7) cDNA sequence (GenBank accession no. L06243), and the reverse primer was a published primer (Kelley et al., 1992). These primers were used to amplify a 1.3-kb fragment from porcine genomic DNA. This fragment included regions corresponding to exon 2, exon 3, and the intron flanked by these two exons. Sequences were obtained from both ends of the PCR fragment and compared with a human sequence showing 95.9% identity at the amino acid level in a 73-amino acid overlap. Sequences produced in this experiment have been submitted to GenBank (accession no. AF052657).

Comments

This is an article from Journal of Animal Science 76 (1998): 2747, doi:/1998.76102747x. Posted with permission.

Copyright Owner
American Society of Animal Science
Language
en
File Format
application/pdf
Citation Information
K. A. Kelly, N. J. Larsen and Max F. Rothschild. "Rapid Communication: Genetic Linkage Mapping of the Porcine Fibroblast Growth Factor 7 (FGF7) Gene" Journal of Animal Science Vol. 76 Iss. 10 (1998) p. 2747 - 2748
Available at: http://works.bepress.com/max-rothschild/93/