Article
Rapid communication: Porcine vitamin D-25-hydroxylase maps to chromosome 5
Journal of Animal Science
Document Type
Article
Disciplines
Publication Version
Published Version
Publication Date
1-1-2000
DOI
/2000.78123193x
Abstract
Locus Name. Vitamin D-25-hydroxylase. Species. Sus scrofa. Locus Symbol. CYP25. Source of Primers. PCR primers were designed from the published sequence for porcine vitamin D-25-hydroxylase (CYP25) cDNA (GenBank accession no. Y16417). The position of the forward and reverse primers corresponds to 506 to 530 bp and 830 to 854 bp, respectively, of the cDNA. The primer pair amplified a 348-bp fragment of the cDNA and an 862-bp fragment from the pig genomic DNA. The 862-bp fragment (Gen- Bank accession no. AF284378) was subcloned into a pNoTA vector and identity was confirmed by sequencing. Genomic sequences from 1 to 74, 169 to 329, and 749 to 862 showed 100% homology to the cDNA.
Copyright Owner
American Society of Animal Science
Copyright Date
2000
Language
en
File Format
application/pdf
Citation Information
Daniel C. Ciobanu, R. K. Gill, Max F. Rothschild and N. H. Bell. "Rapid communication: Porcine vitamin D-25-hydroxylase maps to chromosome 5" Journal of Animal Science Vol. 78 Iss. 12 (2000) p. 3193 - 3194 Available at: http://works.bepress.com/max-rothschild/124/
This is an article from Journal of Animal Science 78 (2000): 3193, doi:/2000.78123193x. Posted with permission.