Genus and Species. Sus scrofa. Locus. Porcine agouti gene. Source and Description of Primers. The forward primer was designed from pig sequence (GenBank accession no. AF018166) and the reverse primer was developed from comparing the homologous regions of mouse and bovine agouti sequences (GenBank accession no. L06451 and X99691, respectively). The primers were used to amplify approximately 1.4 kb of the porcine agouti gene fragment spanning exons 2 and 3. Sequences of the PCR fragment revealed 83% and 89% exonic identities to the corresponding human and bovine agouti nucleotide sequences, respectively. The porcine agouti sequence has been submitted to GenBank, accession no. AF133261.