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Article
Rapid communication: Genetic linkage and physical mapping of the porcine cholesteryl ester transfer protein (CETP) gene
Journal of Animal Science
  • X. W. Shi, Iowa State University
  • Y. D. Zhang, Iowa State University
  • Max F. Rothschild, Iowa State University
  • Christopher K. Tuggle, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
1-1-2002
DOI
/2002.8051390x
Abstract

Source and Description of Primers. The conserved sequences of human and rabbit cholesteryl ester transfer protein gene (CETP) (GenBank accession numbers NM000078 and M27486, respectively) were used to design primers to PCR amplify porcine CETP. The primers amplified a 970-bp fragment of porcine CETP gene spanning an intron between exon 1 and exon 2. The porcine CETP sequence (GenBank accession number AF333037) determined from this fragment showed 80.9%(34/42) and 78.6% (33/42) nucleotide identity to the corresponding human and rabbit CETP exon 2 sequence, respectively. To physically map porcine CETP by PCR testing pig/ rodent somatic cell hybrid panel (SCHP) and to develop PCR-RFLP marker for linkage mapping in PigMaP reference families, a pig-specific reverse primer was designed based on the new generated porcine intron 1 sequence.

Comments

This is an article from Journal of Animal Science 80 (2002): 1390, doi:/2002.8051390x. Posted with permission.

Copyright Owner
American Society of Animal Science
Language
en
File Format
application/pdf
Citation Information
X. W. Shi, Y. D. Zhang, Max F. Rothschild and Christopher K. Tuggle. "Rapid communication: Genetic linkage and physical mapping of the porcine cholesteryl ester transfer protein (CETP) gene" Journal of Animal Science Vol. 80 Iss. 5 (2002) p. 1390 - 1391
Available at: http://works.bepress.com/max-rothschild/113/