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Article
Rapid Communication: TaqI Restriction Fragment Length Polymorphism at the Porcine Bone Morphogenetic Protein 6 (BMPG) Locus
Journal of Animal Science
Document Type
Article
Disciplines
Publication Version
Published Version
Publication Date
1-1-1995
DOI
/1995.731312x
Abstract
Source and Description of Probe. A 1.1-kb human cDNA clone for bone morphogenetic protein 6 (BMP6) was excised from the clone U2-7 (Celeste et al., 1990). Method of Detection. Genomic DNA was isolated from whole blood and digested with TuqI. Fragments were separated by agarose gel electrophoresis and akaline vacuum-transferred to charged nylon membranes. Hybridizations were at 65°C for 16 to 20 h (10% dextran sulfate, .5 M NaC1, .05 M sodium phosphate, pH 6.5, 5x Denhardt's, .5% SDS, 100 pg/ mL sonicated, denatured salmon sperm DNA). Final washes were at 60°C in .7x SSC, 3% SDS for 15 to 20 min
Copyright Owner
American Society of Animal Science
Copyright Date
1995
Language
en
File Format
application/pdf
Citation Information
D. A. Vaske, R. G. Larson, Carol M. Warner and Max F. Rothschild. "Rapid Communication: TaqI Restriction Fragment Length Polymorphism at the Porcine Bone Morphogenetic Protein 6 (BMPG) Locus" Journal of Animal Science Vol. 73 Iss. 1 (1995) p. 312 - 312 Available at: http://works.bepress.com/max-rothschild/110/
This is an article from Journal of Animal Science 73 (1995): 312, doi:/1995.731312x. Posted with permission.