Skip to main content
Article
Rapid Communication: Restriction Fragment Length Polymorphisms at the Porcine Transporter Associated with Antigen Processing 1 (TAP1) Locus
Journal of Animal Science
  • D. A. Vaske, Iowa State University
  • M. K. Ruohonen-Lehto, Iowa State University
  • R. G. Larson, Iowa State University
  • Carol M. Warner, Northeastern University
  • Max F. Rothschild, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
1-1-1994
DOI
/1994.721255x
Abstract

Source and Description of Probe. A 2.7-kb human cDNA clone for TAP1 was excised from the XbaI site of the plasmid pRSV.5neo (Spies et al., 1990). Method of Detection. Hybridizations were performed at 65°C for 16 to 20 h (10% dextran sulfate, 7% SDS, .263 M NazHP04, 1% BSA, 1 mM EDTA, 100 pg/mL sonicated denatured salmon sperm DNA). Final washes were done at 65°C in .7x SSC, .5% SDS for 15 to 20 min.

Comments

This is an article from Journal of Animal Science 72 (1994): 255, doi:/1994.721255x. Posted with permission.

Copyright Owner
American Society of Animal Science
Language
en
File Format
application/pdf
Citation Information
D. A. Vaske, M. K. Ruohonen-Lehto, R. G. Larson, Carol M. Warner, et al.. "Rapid Communication: Restriction Fragment Length Polymorphisms at the Porcine Transporter Associated with Antigen Processing 1 (TAP1) Locus" Journal of Animal Science Vol. 72 Iss. 1 (1994) p. 255 - 255
Available at: http://works.bepress.com/max-rothschild/105/