Poly(ADP-ribose)  metabolism  plays  a  major  role  in  DNA  repair,  transcription,  replication,  and  recombination.  Poly(ADP-ribose)polymerases are localized primarily to the nucleus, whereas significant levels of poly(ADP-ribose) glycohydrolase (PARG) are believed tobe located in the cytoplasm. Only one PARG gene has been identified, but prior studies have reported multiple products of this gene. Herewe studied PARG activity and PARG gene expression in several CNS cell types that span the cell growth spectrum: rapidly dividing C6glioma  tumor  cells,  dividing  astrocytes,  non-dividing  astrocytes  (due  to  contact  inhibition),  and  post-mitotic  neurons.  Activity  assaysshowed no overall differences between these cell types, but the nuclear to cytoplasmic ratio of PARG activity was highest in C6 gliomacells and lowest in neurons. Western blotting revealed full-length PARG as well as lower molecular weight PARG species in all four celltypes.