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Characterization of Humic Acid Size Fractions by SEC and MALS
Organic Geochemistry
  • Ray von Wandruszka, University of Idaho
  • Martin Schimpf, Boise State University
  • Michael Hill, Department of Chemistry and Biochemistry, Boise State Unviersity
  • Regginal Engebretson, University of Idaho
Document Type
Article
Publication Date
4-1-1999
Disciplines
Abstract
Latahco silt-loam humic acid was separated on a preparatory scale by size exclusion chromatography (SEC) on a gravity-fed Sepharose column. Four fractions from this separation were collected and further analyzed, along with whole humic acid, by high-performance SEC coupled with a multi-angle light scattering (MALS) detector. A detailed characterization of the radius of gyration across the elution profile of each fraction by MALS demonstrated that the preparative column generally separated the humic acid according to differences in molecular size. Furthermore, a comparison of elution profiles obtained on the whole humic acid with UV and refractive index detectors revealed the presence of a non-aromatic low molecular weight component.
Citation Information
Ray von Wandruszka, Martin Schimpf, Michael Hill and Regginal Engebretson. "Characterization of Humic Acid Size Fractions by SEC and MALS" Organic Geochemistry (1999)
Available at: http://works.bepress.com/martin_schimpf/32/