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Article
Clusterin facilitates in vivo clearance of extracellular misfolded proteins
Faculty of Science - Papers (Archive)
  • Amy R Wyatt, University of Wollongong
  • Justin J Yerbury, University of Wollongong
  • Paula Berghofer, ANSTO
  • I Greguric, ANSTO
  • Andrew Katsifis, ANSTO
  • Christopher Dobson, University of Cambridge
  • Mark R Wilson, University of Wollongong
RIS ID
43151
Publication Date
1-1-2011
Publication Details

Wyatt, A. R., Yerbury, J., Berghofer, P., Greguric, I., Katsifis, A., Dobson, C. & Wilson, M. R. (2011). Clusterin facilitates in vivo clearance of extracellular misfolded proteins. Cellular and Molecular Life Sciences, 68 (23), 3919-3931.

Abstract
The extracellular deposition of misfolded proteins is a characteristic of many debilitating age-related disorders. However, little is known about the specific mechanisms that act to suppress this process in vivo. Clusterin (CLU) is an extracellular chaperone that forms stable and soluble complexes with misfolded client proteins. Here we explore the fate of complexes formed between CLU and misfolded proteins both in vitro and in a living organism. We show that proteins injected into rats are cleared more rapidly from circulation when complexed with CLU as a result of their more efficient localisation to the liver and that this clearance is delayed by pre-injection with the scavenger receptor inhibitor fucoidan. The CLU-client complexes were found to bind preferentially, in a fucoidan-inhibitable manner, to human peripheral blood monocytes and isolated rat hepatocytes and in the latter cell type were internalized and targeted to lysosomes for degradation. The data suggest, therefore, that CLU plays a key role in an extracellular proteostasis system that recognises, keeps soluble, and then rapidly mediates the disposal of misfolded proteins.
Citation Information
Amy R Wyatt, Justin J Yerbury, Paula Berghofer, I Greguric, et al.. "Clusterin facilitates in vivo clearance of extracellular misfolded proteins" (2011) p. 3919 - 3931
Available at: http://works.bepress.com/mark_wilson/46/