In 1988, data describing the prairie plant germplasm maintained by the U.S. National Plant Germplasm System (NPGS) were surveyed, and the results were reported to the Eleventh North American Prairie Conference. During the 6 years since that initial survey, the taxonomic database of the Germplasm Resources Information Network (GRIN), the primary database for the NPGS, has been improved substantially, and additional germplasm has been added to the NPGS and made available for distribution. Because of this progress, NPGS holdings were surveyed anew, and demand for these holdings was also examined. In 1988, 295 or 862 prairie taxa identified from a search of midwesten floras were included in the GRIN database. By 1994, this number had increased 33% to 393. In 1988, only 146 of these taxa were actually maintained by NPGS, but now 184 taxa are maintained, with 92 currently available to researchers. All taxa held by NPGS that were not included in the initial survey report are listed along with addresses for maintenance sites. Trends in usage relative to other germplasm holdings are also discussed.