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Article
Cryopreservation of Dormant Buds from Diverse Fraxinus Species
CyroLetters
  • Gayle M. Volk, United States Department of Agriculture
  • R. Bonnart, United States Department of Agriculture
  • J. Waddell, United States Department of Agriculture
  • Mark P. Widrlechner, United States Department of Agriculture
Document Type
Article
Publication Date
7-1-2009
Abstract
Ash (Fraxinus) is an economically important tree genus in the landscape industry, as well as a key component of North American forests, especially in the North Central United States and adjacent regions in Canada. In recent years, the Emerald Ash Borer beetle (Agrilus planipennis) has significantly threatened the survival of native North American Fraxinus species. A dormant-bud cryopreservation technique has been developed as a method to conserve specific clones of ash. Dormant buds of three ash species were successfully cryopreserved when desiccated on their stem sections to 30% moisture content (w/v) and then cooled at rates of either −1°C/h or −5°C/day to either −30 or −35°C before immersion in liquid nitrogen vapor (LNV). Stem sections were removed from LNV, warmed, and rehydrated, and their buds grafted onto rootstocks to evaluate survival. Recovery percentages ranged from 34 to 100% after LNV exposure and were dependent upon accession and cooling rate. The cryopreservation methods proposed herein can complement seed-collection efforts aimed at conserving diversity, supplementing ex situ genebank and botanic-garden collections.
Comments

This article is from CyroLetters 30, no. 3 (2009): 262–267

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
Date Available
February 9, 2013
File Format
application/pdf
Citation Information
Gayle M. Volk, R. Bonnart, J. Waddell and Mark P. Widrlechner. "Cryopreservation of Dormant Buds from Diverse Fraxinus Species" CyroLetters Vol. 30 Iss. 4 (2009) p. 262 - 267
Available at: http://works.bepress.com/mark_widrlechner/21/