Transport of L-glucose by Rhodotorula glutinisBiochimie
AbstractThe kinetics of l-glucose transport by Rhodotorula glutinis were studied over a 720-fold range of sugar concentrations. Analysis of the saturation isotherm revealed the presence of a one-carrier system for l-glucose in the plasma membrane of Rhodotorula glutinis. This carrier exhibited a km of 3.7 ± 0.3 mM. d-Ribose was found to be a competitive inhibitor with a Ki of 19 ± 1 mM. The results suggest that l-glucose is transported by the high-Km, d-ribose carrier. l-Glucose was transported against a concentration gradient and the transport was inhibited by the proton conductor 2,4-dinitrophenol.
- rhodotorula glutinis,
- active transport
Citation InformationMark D. Pinkerton, C. K. Ritchie and C. C. Griffin. "Transport of L-glucose by Rhodotorula glutinis" Biochimie Vol. 70 Iss. 2 (1988) p. 183 - 185
Available at: http://works.bepress.com/mark_pinkerton/2/