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Article
Transport of L-glucose by Rhodotorula glutinis
Biochimie
  • Mark D. Pinkerton, Cedarville University
  • C. K. Ritchie
  • C. C. Griffin
Document Type
Article
Publication Date
2-1-1988
DOI
10.1016/0300-9084(88)90059-4
Abstract
The kinetics of l-glucose transport by Rhodotorula glutinis were studied over a 720-fold range of sugar concentrations. Analysis of the saturation isotherm revealed the presence of a one-carrier system for l-glucose in the plasma membrane of Rhodotorula glutinis. This carrier exhibited a km of 3.7 ± 0.3 mM. d-Ribose was found to be a competitive inhibitor with a Ki of 19 ± 1 mM. The results suggest that l-glucose is transported by the high-Km, d-ribose carrier. l-Glucose was transported against a concentration gradient and the transport was inhibited by the proton conductor 2,4-dinitrophenol.
Keywords
  • L-gluclose,
  • sugar,
  • rhodotorula glutinis,
  • active transport
Citation Information
Mark D. Pinkerton, C. K. Ritchie and C. C. Griffin. "Transport of L-glucose by Rhodotorula glutinis" Biochimie Vol. 70 Iss. 2 (1988) p. 183 - 185
Available at: http://works.bepress.com/mark_pinkerton/2/