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Article
Protection against Experimental Autoimmune Myocarditis Is Mediated by Interleukin-10-Producing T Cells that Are Controlled by Dendritic Cells
Faculty Publications and Presentations
  • Ya Li
  • Janet S. Heuser
  • Stanley D. Kosanke
  • Mark E. Hemric, Liberty University
  • Madeleine W. Cunningham
Publication Date
7-1-2005
Disciplines
Comments
Reproduced from Am J Pathol 2005, 167:1 5‐15with permission from the American Society for Investigative Pathology. For more information on the article, go to http://ajp.amjpathol.org/cgi/content/abstract/167/1/5?maxtoshow=&hits=10&RESULTFORMAT=&author1=hemric&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT .
Abstract
Experimental autoimmune myocarditis (EAM) can be induced in the Lewis rat by cardiac myosin or its cryptic S2-16 peptide epitope (amino acids1052 to 1076). To investigate cellular mechanisms and the role of antigen-presenting cells in regulation of myocarditis, we induced protection against EAM in Lewis rats by administration of S2-16 peptide in incomplete Freund’s adjuvant (IFA). Protection to EAM was associated with activation of S2-16-reactive splenocytes secreting high levels of interleukin (IL)-10 and reduced levels of interferon- and IL-2. Adoptive transfer of S2-16:IFA-induced splenocytes producing IL-10 suppressed myocarditis induction in syngeneic recipients, suggesting their regulatory cell nature. However, exposure of S2-16:IFA-induced cells to inflammatory cytokine IL-12 converted them to Th1 effectors that transferred EAM. Differentiated function of S2-16-reactive T cells in protected rats resulted from increased IL-10 production by dendritic cells (DCs). Purified DCs from S2-16:IFA-treated rats promoted S2-16-reactive CD4 T cells to produce increased IL-10 and reduced interferon-. In addition, adoptive transfer of IL-10-producing DCs from S2-16: IFA-treated rats also induced protection to EAM in recipient rats. These studies demonstrated DCs and key cytokines, such as IL-10 and IL-12, regulated the fate of T cells in myocarditis development in the Lewis rat.
Citation Information
Ya Li, Janet S. Heuser, Stanley D. Kosanke, Mark E. Hemric, et al.. "Protection against Experimental Autoimmune Myocarditis Is Mediated by Interleukin-10-Producing T Cells that Are Controlled by Dendritic Cells" (2005)
Available at: http://works.bepress.com/mark_hemric/5/