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Enhanced Surfactant Protein and Defensin mRNA Levels and Reduced Viral Replication during Parainfluenza Virus Type 3 Pneumonia in Neonatal Lambs
Clinical and Diagnostic Laboratory Immunology
  • Branka Grubor, Iowa State University
  • Jack M. Gallup, Iowa State University
  • David K. Meyerholz, Iowa State University
  • Erika C. Crouch, Washington University in St Louis
  • Richard B. Evans, Iowa State University
  • Kim A. Brogden, United States Department of Agriculture
  • Howard D. Lehmkuhl, United States Department of Agriculture
  • Mark R. Ackermann, Iowa State University
Document Type
Article
Publication Date
5-1-2004
DOI
10.1128/CDLI.11.3.599–607.2004
Abstract

Defensins and surfactant protein A (SP-A) and SP-D are antimicrobial components of the pulmonary innate immune system. The purpose of this study was to determine the extent to which parainfluenza type 3 virus infection in neonatal lambs alters expression of sheep beta-defensin 1 (SBD-1), SP-A, and SP-D, all of which are constitutively transcribed by respiratory epithelia. Parainfluenza type 3 viral antigen was detected by immunohistochemistry (IHC) in the bronchioles of all infected lambs 3 days postinoculation and at diminished levels 6 days postinoculation, but it was absent 17 days postinoculation. At all times postinoculation, lung homogenates from parainfluenza type 3 virus-inoculated animals had increased SBD-1, SP-A, and SP-D mRNA levels as detected by fluorogenic real-time reverse transcriptase PCR. Protein levels of SP-A in lung homogenates detected by quantitative-competitive enzyme-linked immunosorbent assay and protein antigen of SP-A detected by IHC were not altered. These studies demonstrate that parainfluenza type 3 virus infection results in enhanced expression of constitutively transcribed innate immune factors expressed by respiratory epithelia and that this increased expression occurs concurrently with decreased viral replication.

Comments

This article is from Clinical and Diagnostic Laboratory Immunology 11, no. 3 (May 2004): 599–607, doi:10.1128/CDLI.11.3.599–607.2004.

Copyright Owner
American Society for Microbiology
Language
en
Date Available
February 25, 2013
File Format
application/pdf
Citation Information
Branka Grubor, Jack M. Gallup, David K. Meyerholz, Erika C. Crouch, et al.. "Enhanced Surfactant Protein and Defensin mRNA Levels and Reduced Viral Replication during Parainfluenza Virus Type 3 Pneumonia in Neonatal Lambs" Clinical and Diagnostic Laboratory Immunology Vol. 11 Iss. 3 (2004) p. 599 - 607
Available at: http://works.bepress.com/mark_ackermann/6/