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Article
Mast cell density and substance P-like immunoreactivity during the initiation and progression of lung lesions in ovineMannheimia (Pasteurella) haemolytica pneumonia
Microbial Pathogenesis
  • Rafael Ramírez-Romero, Iowa State University
  • Kim A. Brogden, United States Department of Agriculture
  • Jack M. Gallup, Iowa State University
  • Ioana M. Sonea, Iowa State University
  • Mark R. Ackermann, Iowa State University
Document Type
Article
Publication Date
6-1-2001
DOI
10.1006/mpat.2000.0437
Abstract

To determine the density of mast cells (MCs) and the extent of substance P (SP) immunoreactivity during initiation and progression of pneumonic pasteurellosis (PP), 18 lambs were inoculated intrabronchially withMannheimia (Pasteurella) haemolytica or saline, and lung tissue was collected at 1, 15 and 45 days post-inoculation (n=3, each group). Additionally, the left (non-inoculated) contralateral lungs in bacteria-inoculated animals were collected as controls. At 1 day after bacterial inoculation the lungs had typical M. haemolyticalesions. These pneumonic lesions had fewer numbers of MCs and reduced histamine content. Macrophages infiltrating some of the inflamed areas were strongly immunoreactive for SP. At 15 days, MCs remained scarce at sites where lung damage persisted, i.e. pyogranulomatous foci, but were increased in number in areas of interstitial damage. Pulmonary ganglion neurons were strongly immunoreactive for SP. By 45 days the fibrosing changes became more defined as pleural fibrosis, fibrosing alveolitis, alveolar epithelial hyperplasia and bronchiolitis obliterans. These lungs had increased numbers of MCs, but histamine content was not different from saline- and non-inoculated left lungs. Substance P immunoreactivity occurred only in nerves and was scarce and mild. This work demonstrates that MC density decreases initially with PP, but increases with progression of PP. SP fibres tend to be decreased during the initiation and at 45 days of PP, but other cells, such as macrophages and neuronal ganglion cells, produce substance P during progression of PP and thereby constitute an additional source of substance P.

Comments

This article is from Microbial Pathogenesis 30, no. 6 (June 2001): 325–335, doi:10.1006/mpat.2000.0437.

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
Date Available
February 26, 2013
File Format
application/pdf
Citation Information
Rafael Ramírez-Romero, Kim A. Brogden, Jack M. Gallup, Ioana M. Sonea, et al.. "Mast cell density and substance P-like immunoreactivity during the initiation and progression of lung lesions in ovineMannheimia (Pasteurella) haemolytica pneumonia" Microbial Pathogenesis Vol. 30 Iss. 6 (2001) p. 325 - 335
Available at: http://works.bepress.com/mark_ackermann/46/