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Article
Comparison of bronchoalveolar lavage fluid obtained from Mannheimia haemolytica-inoculated calves with and without prior treatment with the selectin inhibitor TBC1269
American Journal of Veterinary Research
  • Jessica M. Caverly, Iowa State University
  • Zaher A. Radi, Iowa State University
  • Claire B. Andreasen, Iowa State University
  • Richard A. Dixon, Texas Biotechnology Corporation
  • Kim A. Brogden, United States Department of Agriculture
  • Mark R. Ackermann, Iowa State University
Document Type
Article
Publication Date
5-1-2001
DOI
10.2460/ajvr.2001.62.665
Abstract

Objectives—To determine effects of selectin inhibitor TBC1269 on neutrophil infiltration, and neutrophilassociated injury during pneumonia induced byMannheimia haemolytica and concentration of antimicrobial anionic peptide (AAP) in bronchoalveolar lavage fluid (BALF) as well as antimicrobial activity of BALF from healthy (control) neonatal calves, neonatal calves with M haemolytica-induced pneumonia, neonatal calves with prior treatment with TBC1269, and adult cattle.

Animals—Eighteen 1- to 3-day-old calves and 9 adult cattle.

Procedure—Calves were inoculated with M haemolyticaor pyrogen-free saline (0.14M NaCl) solution into the right cranial lung lobe, and BALF was collected 2 or 6 hours after inoculation. Thirty minutes before and 2 hours after inoculation, 4 calves received TBC1269. The BALF collected from 9 adult cattle was used for comparison of BALF AAP concentration and antimicrobial activity. Protein concentration and neutrophil differential percentage and degeneration in BALF were determined. An ELISA and killing assay were used to determine BALF AAP concentration and antimicrobial activity, respectively.

Results—Total protein concentration was significantly decreased in BALF from calves receiving TBC1269. Similar concentrations of AAP were detected in BALF from all calves, which were 3-fold higher than those in BALF from adult cattle. However, BALF from neonates had little or no anti-M haemolytica activity.

Conclusions and Clinical Relevance—These results suggest that TBC1269 decreases pulmonary tissue injury in neonatal calves infected with M haemolytica. Although AAP is detectable in neonatal BALF at 3 times the concentration detected in adult BALF, neonatal BALF lacks antimicrobial activity forM haemolytica.

Comments

This article is from American Journal of Veterinary Research 62, no. 5 (May 2001): 665–672, doi:10.2460/ajvr.2001.62.665.

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
Date Available
February 26, 2013
File Format
application/pdf
Citation Information
Jessica M. Caverly, Zaher A. Radi, Claire B. Andreasen, Richard A. Dixon, et al.. "Comparison of bronchoalveolar lavage fluid obtained from Mannheimia haemolytica-inoculated calves with and without prior treatment with the selectin inhibitor TBC1269" American Journal of Veterinary Research Vol. 62 Iss. 5 (2001) p. 665 - 672
Available at: http://works.bepress.com/mark_ackermann/43/