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Article
Early Epithelial Invasion by Salmonella enterica Serovar Typhimurium DT104 in the Swine Ileum
Veterinary Pathology
  • David K. Meyerholz, Iowa State University
  • Thomas J. Stabel, United States Department of Agriculture
  • Mark R. Ackermann, Iowa State University
  • S. A. Carlson, United States Department of Agriculture
  • B. D. Jones, University of Iowa
  • J. Pohlenz, Tierärztliche Hochschule Hannover
Document Type
Article
Publication Date
11-1-2002
DOI
10.1354/vp.39-6-712
Abstract

Salmonella enterica serovar Typhimurium is an important intestinal pathogen in swine. This study was performed to document the early cellular invasion of Salmonellaserovar Typhimurium in swine ileum. Ileal gut-loops were surgically prepared in ten 4- to 5-week-old mixed-breed pigs and inoculated for 0-60 minutes. Loops were harvested and prepared for both scanning and transmission electron microscopy (SEM and TEM, respectively). Preferential bacterial adherence to microfold cells (M cells) was seen within 5 minutes, and by 10 minutes bacterial invasion of the apical membrane was seen in M cells, goblet cells, and enterocytes. This multicellular invasion was observed throughout the course of infection. In addition, SEM revealed a specific affinity of Salmonella serovar Typhimurium to sites of cell extrusion. Using TEM, bacteria in these areas were focused in the crevices formed by the extruding cell and the adjacent cells and in the cytoplasm immediately beneath the extruding cell. Our results suggest that early cellular invasion by Salmonella serovar Typhimurium is nonspecific and rapid in swine. Furthermore, the combination of SEM and TEM data suggests that Salmonella serovar Typhimurium may use sites of cell extrusion as an additional mechanism for early invasion.

Comments

This article is from Veterinary Pathology 39, no. 6 (November 2002): 712–720, doi:10.1354/vp.39-6-712.

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
Date Available
February 26, 2013
File Format
application/pdf
Citation Information
David K. Meyerholz, Thomas J. Stabel, Mark R. Ackermann, S. A. Carlson, et al.. "Early Epithelial Invasion by Salmonella enterica Serovar Typhimurium DT104 in the Swine Ileum" Veterinary Pathology Vol. 39 Iss. 6 (2002) p. 712 - 720
Available at: http://works.bepress.com/mark_ackermann/37/