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Article
A selectin inhibitor decreases neutrophil infiltration during acute Mannheimia haemolytica pneumonia
Veterinary Pathology
  • Zaher A. Radi, Iowa State University
  • Kim A. Brogden, United States Department of Agriculture
  • Richard A. Dixon, Texas Biotechnology Corporation
  • Jack M. Gallup, Iowa State University
  • Mark R. Ackermann, Iowa State University
Document Type
Article
Publication Date
11-1-2002
DOI
10.1354/vp.39-6-697
Abstract

The degree to which the selectin inhibitor TBC1269 reduces neutrophil infiltration in specific microscopic locations of the lung during acute pneumonia of neonates was determined. Neonatal calves were inoculated intrabronchially with Mannheimia (Pasteurella) haemolytica or saline, and lung tissue was collected at 2 and 6 hours postinoculation (PI). One 6-hour group inoculated with M. haemolytica received TBC1269 intravenously before and after inoculation with M. haemolytica. Infiltrates of neutrophils were significantly higher in the alveolar lumen and septae but lower in the bronchial lumen and epithelium at 6 hours PI than at 2 hours PI. Significantly fewer neutrophils (P > 0.05) were present in the alveolar lumen and septae, and the bronchiolar lumen and lamina propria in the lungs of TBC1269-treated calves compared with untreated calves at 6 hours PI. TBC1269 did not alter the infiltration into bronchi and blood vessels or the expression of the selectin-independent adhesion molecule, ICAM-1. This work suggests that during acute pneumonia of neonates 1) neutrophil infiltrates progressively increase in the alveolar lumens and septae but decrease in the bronchial lumen and epithelium with time, 2) TBC1269 reduces neutrophil infiltration into specific regions of alveoli and bronchioles rather than uniformly throughout the lung, and 3) selectin inhibition does not affect the location and intensity of ICAM-1 expression.

Comments

This article is from Veterinary Pathology, 39, no. 6 (November 2002): 697–705, doi:10.1354/vp.39-6-697.

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
Date Available
February 26, 2013
File Format
application/pdf
Citation Information
Zaher A. Radi, Kim A. Brogden, Richard A. Dixon, Jack M. Gallup, et al.. "A selectin inhibitor decreases neutrophil infiltration during acute Mannheimia haemolytica pneumonia" Veterinary Pathology Vol. 39 Iss. 6 (2002) p. 697 - 705
Available at: http://works.bepress.com/mark_ackermann/28/