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Article
Differential expression of cytokine transcripts in neonatal and adult ovine alveolar macrophages in response to respiratory syncytial virus or toll-like receptor ligation
Veterinary Immunology and Immunopathology
  • Sasha J. Fach, Iowa State University
  • Alicia Kathleen Olivier, Iowa State University
  • Jack M. Gallup, Iowa State University
  • Theresa E. Waters, United States Department of Agriculture
  • Mark R. Ackermann, Iowa State University
  • Howard D. Lehmkuhl, United States Department of Agriculture
  • Randy E. Sacco, United States Department of Agriculture
Document Type
Article
Publication Date
7-1-2010
DOI
10.1016/j.vetimm.2010.02.008
Abstract
Alveolar macrophages (AMϕs) secrete regulatory molecules that are believed to be critical in maintaining normal lung homeostasis. However, in response to activating signals, AMϕs have been shown to become highly phagocytic cells capable of secreting significant levels of pro-inflammatory cytokines. There is evidence to suggest that susceptibility of Mϕ subpopulations to viral infection, and their subsequent cytokine/chemokine response, is dependent on age of the host. In the present study, we compared bovine respiratory syncytial virus (BRSV) replication and induction of cytokine responses in neonatal ovine AMϕs to those cells isolated from adult animals. While neonatal AMϕs could be infected with BRSV, viral replication was limited as previously shown for AMϕs from mature animals. Interestingly, following BRSV infection, peak mRNA levels of IL-1β and IL-8 in neonatal AMϕ were several fold higher than levels induced in adult AMϕs. In addition, peak mRNA expression for the cytokines examined occurred at earlier time points in neonatal AMϕs compared to adult AMϕs. However, the data indicated that viral replication was not required for the induction of specific cytokines in either neonatal or adult AMϕs. TLR3 and TLR4 agonists induced significantly higher levels of cytokine transcripts than BRSV in both neonatal and adult AMϕs. It was recently proposed that immaturity of the neonatal immune system extends from production of pro-inflammatory cytokines to regulation of such responses. Differential regulation of cytokines in neonatal AMϕs compared to adult AMϕs in response to RSV could be a contributory factor to more severe clinical episodes seen in neonates.
Comments

This article is from Veterinary Immunology and Immunopathology 136, no. 1–2 (July 2010): 55–64, doi:10.1016/j.vetimm.2010.02.008.

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
Date Available
February 25, 2013
File Format
application/pdf
Citation Information
Sasha J. Fach, Alicia Kathleen Olivier, Jack M. Gallup, Theresa E. Waters, et al.. "Differential expression of cytokine transcripts in neonatal and adult ovine alveolar macrophages in response to respiratory syncytial virus or toll-like receptor ligation" Veterinary Immunology and Immunopathology Vol. 136 Iss. 1–2 (2010) p. 55 - 64
Available at: http://works.bepress.com/mark_ackermann/2/