Tillage system and crop rotation have long-term effects on soil productivity and soil quality components such as soil carbon as well as on soil physical, biological, and chemical properties. In addition, both tillage and crop rotation have effects on weed and soil disease control. There is a need for well-defined, long term tillage and crop rotation studies across the different soils and climate conditions in Iowa. The objective of this study was to evaluate the long-term effects of different tillage systems and crop rotations on soil productivity.